Journal: Chinese Journal of Analytical Chemistry

Article Title: Active substances and molecular mechanisms of Astragali Radix and Angelicae Sinensis Radix against idiopathic pulmonary fibrosis effects by network pharmacology and in vitro experiments

doi: 10.1016/j.cjac.2024.100397

Figure Lengend Snippet: Fig. 7. Molecular docking results of main components of AR and ASR. (A) Quercetin - IL-6; (B) magnolol - JAK2; (C) riligustilide - JAK2; (D) coniferyl ferulate - STAT3; (E) valerophenone - STAT3.

Article Snippet: The primary antibodies in western blot were as follows: Anti ‐α- MA (catalog number: 19,245), anti ‐STAT3 (catalog number: 12640S), nti ‐phospho ‐STAT3 (catalog number: 9145S), anti ‐total ‐JAK2 (catalog umber: 3230S), anti ‐phospho ‐JAK2 (catalog number: 3771S) antibodes were obtained from Cell Signaling Technology (Danvers, MA).

Techniques:

Journal: Chinese Journal of Analytical Chemistry

Article Title: Active substances and molecular mechanisms of Astragali Radix and Angelicae Sinensis Radix against idiopathic pulmonary fibrosis effects by network pharmacology and in vitro experiments

doi: 10.1016/j.cjac.2024.100397

Figure Lengend Snippet: Fig. 9. Effects of quercetin and magnolol on the JAK2/STAT3 signaling pathway in the A549 cells. Data are expressed as mean ± tan- dard error of the mean (SEM) ( n = 3/group). # p < 0.05 vs. Con group. ## p < 0.01 vs. Con group. ∗ p < 0.05 vs. IL-6 group. ∗ ∗ p < 0.01 vs. IL-6 group. △p < 0.05 vs. Magnolol group. △△p < 0.01 vs. Magnolol group.

Article Snippet: The primary antibodies in western blot were as follows: Anti ‐α- MA (catalog number: 19,245), anti ‐STAT3 (catalog number: 12640S), nti ‐phospho ‐STAT3 (catalog number: 9145S), anti ‐total ‐JAK2 (catalog umber: 3230S), anti ‐phospho ‐JAK2 (catalog number: 3771S) antibodes were obtained from Cell Signaling Technology (Danvers, MA).

Techniques:

Journal: Chinese Journal of Analytical Chemistry

Article Title: Active substances and molecular mechanisms of Astragali Radix and Angelicae Sinensis Radix against idiopathic pulmonary fibrosis effects by network pharmacology and in vitro experiments

doi: 10.1016/j.cjac.2024.100397

Figure Lengend Snippet: Fig. 10. The mRNA expression of the JAK2/STAT3 signaling path- way in the A549 cells. (A) HIF-1 𝛼mRNA expression. (B) 𝛼-SAM mRNA expression. Data are expressed as mean ± SEM ( n = 6/group) #p < 0.05 vs. Con group. ## p < 0.01 vs. Con group. ∗ p < 0.05 vs. IL-6 group. ∗ ∗ p < 0.01 vs. IL-6 group. △p < 0.05 vs. Magnolol group. △△p < 0.01 vs. Magnolol group.

Article Snippet: The primary antibodies in western blot were as follows: Anti ‐α- MA (catalog number: 19,245), anti ‐STAT3 (catalog number: 12640S), nti ‐phospho ‐STAT3 (catalog number: 9145S), anti ‐total ‐JAK2 (catalog umber: 3230S), anti ‐phospho ‐JAK2 (catalog number: 3771S) antibodes were obtained from Cell Signaling Technology (Danvers, MA).

Techniques: Expressing

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: miR-34b Alleviates High Glucose-Induced Inflammation and Apoptosis in Human HK-2 Cells via IL-6R/JAK2/STAT3 Signaling Pathway

doi: 10.12659/MSM.917128

Figure Lengend Snippet: miR-34b suppresses activation of the JAK2/STAT3 signaling pathway. ( A, B ) The p-STAT3, t-STAT3, p-JAK2, and t- JAK2 expression in HG-treated HK-2 cells tranfected with miR-34b mimic or controls. ( C, D ) The p-STAT3, t-STAT3, p-JAK2, and t-JAK2 expression in HG-treated HK-2 cells co-transfected with pcDNA-IL-6R, empty vector, miR-34b mimic, or mimic-NC. Data are presented as mean ±SD and shown as fold change relative to the control group. Data were assessed with one-way ANOVA. * p<0.05, ** p<0.01, and *** p<0.001. p-STAT3 – phosphorylated STAT3; p-JAK2 – phosphorylated JAK2; t-STAT3 – total STAT3; t-JAK2 – total JAK2; HG – high glucose; NG – normal glucose.

Article Snippet: The primary polyclonal antibodies were: caspase-3; TNF-α, (1: 200, rabbit; Abcam); phosphorylated-STAT3 (p-STAT3), phosphorylated-JAK2 (p-JAK2), total STAT3 (t-STAT3), total JAK2 (t-JAK2) (1: 500, rabbit; Abcam); and IL-1β and IL-6 (1: 3000, rabbit; CA, USA).

Techniques: Activation Assay, Expressing, Transfection, Plasmid Preparation